GV-PLT-TOCV · Virus
Tomato Chlorosis Virus (ToCV) Detection Kit
High-Sensitivity Field Nucleic Acid Test Kit
Target: Tomato chlorosis virus (ToCV).
IFU sample types: seeds, leaves, and fruits.
Product overview
GeneVide ToCV Tomato Chlorosis Virus On-Site Plant Test Kit · GV-PLT-TOCV
This kit amplifies nucleic acid sequences specific to Tomato Chlorosis Virus (ToCV) and uses directional signal amplification technology to achieve high-sensitivity detection of the target nucleic acid. Applicable sample types: seeds, leaves, and fruits.
Package specification in the IFU
16 tests/box
The IFU describes the individual box. Confirm the current sales-pack quantity, price, shipping, and availability on the linked eBay listing before purchasing; an order may contain multiple boxes.
Test principle
This kit amplifies nucleic acid sequences specific to Tomato Chlorosis Virus (ToCV) and uses directional signal amplification technology to achieve high-sensitivity detection of the target nucleic acid.
Kit components
Table 1. Main components
| No. | Component | Main ingredients | Specification | Qty. |
|---|---|---|---|---|
| 1 | Sample soaking solution | Buffer | 60 mL/bottle | 1 |
| 2 | Zip bags | — | 16 pcs/bag | 1 |
| 3 | ToCV enzyme pellet | Primers, probe, endonuclease, deoxyribonucleoside triphosphates, polymerase | 16 tests/pack | 1 |
| 4 | ToCV positive control | Target plasmid containing the target fragment | 280 μL/tube | 1 |
| 5 | Negative control | Nuclease-free water | 280 μL/tube | 1 |
| 6 | Lysis solution | Buffer, resin | 16 tubes/bag | 1 |
Storage and shelf life
Store at 2–8°C. Shelf life: 12 months.
Workflow and result interpretation
These instructions are specific to Tomato Chlorosis Virus (ToCV). Follow the full IFU, including controls and precautions. Reaction thresholds are not total sample-to-result times.
Test Procedure
- Remove and cut off the required number of enzyme-pellet tubes. Along the wall of separate tubes, add 50 μL of negative control, nucleic acid solution for testing (use the supernatant and avoid solids at the bottom), and positive control, respectively.
- Close the tube caps tightly. Hold both ends of each tube between the thumb and index finger, mix forcefully up and down 10 times, and then flick downward forcefully 3 times to collect the liquid at the bottom. Visually confirm that the enzyme pellet is completely dissolved, the liquid level is uniform, and no bubbles remain at the bottom; then place the tubes in the palm-sized fluorescence detector for testing.
Note: Manual mixing is a critical step and must be performed thoroughly. Before starting the test, make sure the enzyme-pellet tube caps and the lid of the palm-sized fluorescence detector are closed tightly.
Result Interpretation
At the end of the test program, the palm-sized fluorescence detector automatically analyzes and determines the result.
Table 2. Result interpretation for the palm-sized fluorescence detector
| Assessment | TT display | Displayed result |
|---|---|---|
| Negative control | — | NC |
| Positive control | TT ≤ 10 | PC |
| Positive sample | TT ≤ 20 | Positive |
| Negative sample | — | Negative |
Equipment and Consumables Required but Not Provided
- Personal protective equipment (disposable gloves, laboratory coat, protective eyewear, etc.).
- Timer.
- Liquid-waste container.
Precautions
- Do not use expired reagents.
- Perform the entire procedure in a nuclease-free environment.
- Wear disposable gloves during operation.
- Use filtered pipette tips to prevent contamination of the pipette.
- All sample tubes, centrifuge tubes, tips, reagents, and test tubes are single-use and must not be reused.
- Do not mix reagents from different product lots.
Instructions for Use (IFU)
IFU revision: August 5, 2026. English translation based on the corresponding Chinese instruction sheet.
Download English IFUChinese IFU (PDF)
Can I use a different sample type?
The stated sample types are seeds, leaves, and fruits. Contact the product team before using an unlisted sample or modifying the procedure.
Does this kit include the detector?
No. The portable platform is listed separately. Consult the components table and IFU for what is supplied with the reagent kit.
Selection and purchasing questions
Should I select ToCV or TYLCV from a short symptom description?
Confirm the required target first. This product is for Tomato chlorosis virus, while TYLCV is a separate product. A short symptom description is not sufficient to establish which assay your testing plan requires.
What should I include in a ToCV Test Kit inquiry?
Include SKU GV-PLT-TOCV, your actual specimen (the IFU lists seeds, leaves, and fruits), testing volume, existing equipment and destination. Confirm the sales-pack quantity separately from the individual-box specification in the IFU.
Discuss your testing requirements
Include your target, sample type, expected testing volume, and destination. This opens your email application; nothing is submitted until you send the email.
Request a quote by emailNeed help choosing? Use Ask GeneVide AI for product information; confirm critical decisions with the product team.