GV-PLT-ZC · Insect
Melon Fly Detection Kit
High-Sensitivity Field Nucleic Acid Test Kit
Target: Zeugodacus cucurbitae.
IFU sample types: insect specimens; follow the specimen preparation in the IFU.
Product overview
GeneVide Zeugodacus cucurbitae Melon Fly On-Site Test Kit · GV-PLT-ZC
This kit amplifies nucleic acid sequences specific to Melon Fly and uses directional signal amplification technology to achieve high-sensitivity detection of the target nucleic acid.
Package specification in the IFU
16 tests/box
The IFU describes the individual box. Confirm the current sales-pack quantity, price, shipping, and availability on the linked eBay listing before purchasing; an order may contain multiple boxes.
Test principle
This kit amplifies nucleic acid sequences specific to Melon Fly and uses directional signal amplification technology to achieve high-sensitivity detection of the target nucleic acid.
Kit components
Table 1. Main components
| No. | Component | Main ingredients | Specification | Qty. |
|---|---|---|---|---|
| 1 | Melon Fly enzyme pellet | Primers, probe, endonuclease, deoxyribonucleoside triphosphates, polymerase | 16 tests/pack | 1 |
| 2 | Melon Fly positive control | Target plasmid containing the target fragment | 280 μL/tube | 1 |
| 3 | Negative control | Nuclease-free water | 280 μL/tube | 1 |
Storage and shelf life
Store the test kit at 2–8°C. Shelf life: 12 months.
Workflow and result interpretation
These instructions are specific to Melon Fly. Follow the full IFU, including controls and precautions. Reaction thresholds are not total sample-to-result times.
Test Procedure
- Remove and cut off the required number of enzyme-pellet tubes. Along the wall of separate tubes, add 50 μL of negative control, nucleic acid solution for testing, and positive control, respectively. After all solutions have been added, visually confirm that the solution has completely dissolved the enzyme pellet.
- Close the tube caps. Hold both ends of each tube between the thumb and index finger, mix up and down for 10 cycles, and flick gently 3 times to collect the liquid at the bottom. Visually confirm that the liquid level is uniform and no bubbles remain at the bottom; then place the tubes in the palm-sized fluorescence detector for testing.
Note: Manual mixing is a critical step. Before starting the test, make sure the enzyme-pellet tube caps and the lid of the palm-sized fluorescence detector are closed tightly.
Result Interpretation
At the end of the test program, the palm-sized fluorescence detector automatically analyzes and determines the result.
Table 2. Result interpretation for the palm-sized fluorescence detector
| Assessment | TT display | Displayed result |
|---|---|---|
| Negative control | — | NC |
| Positive control | TT ≤ 10 | PC |
| Positive sample | TT ≤ 20 | Positive |
| Negative sample | — | Negative |
Equipment and Consumables Required but Not Provided
- Personal protective equipment (disposable laboratory gloves, laboratory coat, protective eyewear, etc.).
- Timer.
- Liquid-waste container.
Precautions
- Do not use expired reagents.
- Perform the entire procedure in a nuclease-free environment.
- Wear powder-free gloves when handling reagents and nucleic acids.
- To avoid cross-contamination, filtered pipette tips are recommended.
- All sample tubes, centrifuge tubes, tips, reagents, and test tubes are single-use.
- Do not mix reagents from different product lots.
Instructions for Use (IFU)
IFU revision: August 13, 2026. English translation based on the corresponding Chinese instruction sheet.
Download English IFUChinese IFU (PDF)
Can I use a different sample type?
The stated sample types are insect specimens; follow the specimen preparation in the IFU. Contact the product team before using an unlisted sample or modifying the procedure.
Does this kit include the detector?
No. The portable platform is listed separately. Consult the components table and IFU for what is supplied with the reagent kit.
Selection and purchasing questions
Does a melon fly test use the same specimen as a melon leaf test?
Do not infer a plant sample from the common name. This is the Zeugodacus cucurbitae product; confirm the insect specimen and nucleic acid preparation with the corresponding IFU.
What should I include in a Melon Fly Detection Kit inquiry?
Include SKU GV-PLT-ZC, your actual specimen (the IFU lists insect specimens; follow the specimen preparation in the IFU), testing volume, existing equipment and destination. Confirm the sales-pack quantity separately from the individual-box specification in the IFU.
Discuss your testing requirements
Include your target, sample type, expected testing volume, and destination. This opens your email application; nothing is submitted until you send the email.
Request a quote by emailNeed help choosing? Use Ask GeneVide AI for product information; confirm critical decisions with the product team.